Selection of reference genes for quantitative real-time PCR in Cocos nucifera during abiotic stress
作者： Wei Xia , Zheng Liu , Yaodong Yang , Yong Xiao , Annaliese S. Mason
关键词: Botany 、 Gene 、 Gene expression 、 Polymerase chain reaction 、 Reference genes 、 Transcriptome 、 Biology 、 Endosperm 、 Abiotic stress 、 Cocos nucifera
摘要: Reverse transcription quantitative real-time polymerase chain reaction (PCR) is a widely used and reproducible method for studying gene expression changes. However, its accuracy reliability highly dependent on the normalization step. Cocos nucifera L., perennial palm with long productive life span, frequently exposed to soil atmospheric drought other stresses. In applying analysis understand coconut stress responses, validation of suitable reference genes an important first this study, seven putative were identified from transcriptome data. The stability these was assessed in diverse set 18 samples subject cold, drought, high-salinity treatment representing different endosperm developmental stages. Using statistical algorithms geNorm, NormFinder, BestKeeper, eEF1-α UBC10 as stable all treatments development st...
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