A flexible microprocessor system for the measurement of cell size.

作者: J M Round , D A Jones , R H Edwards

DOI: 10.1136/JCP.35.6.620

关键词: Cytological TechniquesBiomedical engineeringPerimeterHistogramStatistical analysesForm factor (electronics)Cell sizeMathematicsMicroprocessor systemAnatomy

摘要: A flexible system for the measurement of length and area is described. The consists Reichert Jung MOP-1 measuring device interfaced with a Commodore PET computer. Its use illustrated by planimetric cross sectional areas in histochemical preparations normal diseased muscle. While measurements are being made data can be displayed on computer screen either numerical form or as frequency histogram together simple statistical analyses. Hard copy obtained from an attached printer. Mean values fibre human skeletal muscle reported. An alternative, widely used method calculating lesser diameter was found to give consistent underestimate approximately 30% when compared our method. In abnormally shaped fibres discrepancy even larger; such identified using "form factor" which relates cell its perimeter. This rapid, accurate also suitable many different types graphical record.

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