A barley activation tagging system.

摘要: Activation tagging, as the result of random genomic insertion either promoter or enhancer sequences, can produce novel, dominant mutations by over-expression endogenous genes. This powerful genomics tool has been used extensively in dicot species such Arabidopsis, while rice is only cereal for which an equivalent system exists. In this study we describe activation tagging barley based upon maize Ac/Ds transposable element system. A modified Ds (UbiDs) containing two polyubiquitin promoters, transposed families derived from multiple independent UbiDs transformants and generated new events at frequencies ranging 0% to 52% per family. The majority elements activated high levels adjacent flanking sequence transcription. Transposon-mediated expression was detected all cell tissue types analysed suggesting that applicable aspects plant development biogenesis. addition transcriptional activation, also capable generating insertional knockout mutants a inactivated allele granule bound starch synthase I gene (waxy) recovered lead reduced amylose accumulation. recovery analysis phenotypes will provide novel approach understanding function large genomes where redundancy may mask conventional loss-of-function mutations.