Alterative Expression and Localization of Profilin 1/VASPpS157 and Cofilin 1/VASPpS239 Regulates Metastatic Growth and Is Modified by DHA Supplementation.

摘要: Profilin 1, cofilin and vasodialator-stimulated phosphoprotein (VASP) are actin-binding proteins (ABP) that regulate actin remodeling facilitate cancer cell metastases. miR-17-92 is highly expressed in metastatic tumors profilin1 cofilin1 predicted targets. Docosahexaenoic acid (DHA) inhibits proliferation adhesion. These studies tested the hypothesis phenotype driven by changes ABPs including alternative phosphorylation and/or subcellular localization. In addition, we efficacy of DHA supplementation to attenuate or inhibit these changes. Human lung tissue sections were analyzed for F-actin content expression cellular localization profilin1, cofilin1, VASP (S157 S239 phosphorylation). The was investigated A549 MLE12 cells lines using 8 Br-cAMP as a metastasis inducer therapeutic agent. Migration assessed wound assay measured Western blot confocal analysis. qRT-PCR. Results indicated increased altered distribution profilin1/VASP(pS157), but no cofilin1/VASP(pS239) human malignant tissues compared with normal tissues. cells, patterns profilin1/VASP(pS157) suggested an interaction regulation dynamics. Furthermore, inhibited migration viability, ABP localization, modulated minimal effects cells. Further investigations warranted understand interactions, miR-17-92, novel therapeutic. Mol Cancer Ther; 15(9); 2220-31. ©2016 AACR.