Exosomes secreted by chronic hepatitis B patients with PNALT and liver inflammation grade ≥ A2 promoted the progression of liver cancer by transferring miR-25-3p to inhibit the co-expression of TCF21 and HHIP.

摘要: Objectives The current study aimed to investigate the mechanism by which exosomes secreted CHB patients with PNALT and liver inflammation grade (≥A2) affected development of cancer. Materials methods Gene expression was assessed RT-PCR, Western blotting immunohistochemistry. CCK-8, colony formation, transwell, scratch-wound flow cytometry assays were used detect cell viability, proliferation, apoptosis metastasis. interaction TCF21 HHIP co-immunoprecipitation assay. Luciferase reporter combination TCF21/HHIP miR-25-3p. Xenograft studies in nude mice manifested tumour growth ability Bioinformatics analyses conducted using TargetScan, EVmiRNA, TCGA, GEO, DAVID, COEXPEDIA, UALCAN, UCSC Human Protein Atlas databases. Results CHB-PNALT-Exo promoted proliferation metastasis HepG2.2.15 cells. miR-25-3p upregulated (≥A2). overexpression related poor survival CHB-PNALT proliferation- metastasis-promoting functions abolished inhibitors. directly interacted HHIP. Inhibition or Knockdown counteracted effects containing inhibitor on Ki67, E-cadherin caspase-3/-9. Conclusions Transfer cancer inhibiting co-expression