Characterization of multiple charge isomers of the inhibitor protein of the cyclic AMP-dependent protein kinase from bovine heart and rabbit skeletal muscle
作者： Susan Whitehouse , John M. McPherson , Donal A. Walsh
DOI: 10.1016/0003-9861(80)90233-7
关键词: Sodium dodecyl sulfate 、 Size-exclusion chromatography 、 Trichloroacetic acid 、 Gel electrophoresis 、 Isoelectric focusing 、 Cardiac muscle 、 Biochemistry 、 Protein kinase A 、 Chromatography 、 Chemistry 、 Inhibitor protein
摘要: Abstract The presence of multiple forms the inhibitor cyclic AMP-dependent protein kinase has been investigated in rabbit skeletal and bovine cardiac muscle. These tissues have each fractionated by two separate procedures, one involving mild techniques, other utilizing trichloroacetic acid precipitation heating to 95 °C. proteins partially purified preparations characterized DEAE-cellulose chromatography, gel filtration, isoelectric focusing, sodium dodecyl sulfate nondenaturing electrophoresis. In tissue three charge isomers detected. Each isomer may exist apparent molecular size designated I I′ ( J. M. McPherson, S. Whitehouse, D. A. Walsh, 1979, Biochemistry 18 , 4835–4845 ). distribution between depends on purification procedure used potentially only form tissues.
elsevier.com
sci-hub.se 参考文章(18)
ARTHUR B. ROBINSON, COLETTE J. RUDD, Deamidation of Glutaminyl and Asparaginyl Residues in Peptides and Proteins Current Topics in Cellular Regulation. ,vol. 8, pp. 247- 295 ,(1974) , 10.1016/B978-0-12-152808-9.50013-4
C. Dennis Ashby, Donal A. Walsh, Characterization of the Interaction of a Protein Inhibitor with Adenosine 3',5'-Monophosphate-dependent Protein Kinases II. MECHANISM OF ACTION WITH THE HOLOENZYME Journal of Biological Chemistry. ,vol. 248, pp. 1255- 1261 ,(1972) , 10.1016/S0021-9258(19)44290-7
Thomas E. Donnelly, J.F. Kuo, Procerfina L. Reyes, Yung-Pin Liu, Paul Greengard, Protein Kinase Modulator from Lobster Tail Muscle Journal of Biological Chemistry. ,vol. 248, pp. 190- 198 ,(1973) , 10.1016/S0021-9258(19)44461-X
A Szmigielski, A Guidotti, E Costa, Endogenous protein kinase inhibitors. Purification, characterization, and distribution in different tissues. Journal of Biological Chemistry. ,vol. 252, pp. 3848- 3853 ,(1977) , 10.1016/S0021-9258(17)40329-2
Thomas E. Donnelly, J.F. Kuo, Eishichi Miyamoto, Paul Greengard, Protein Kinase Modulator from Lobster Tail Muscle Journal of Biological Chemistry. ,vol. 248, pp. 199- 203 ,(1973) , 10.1016/S0021-9258(19)44462-1
C. Dennis Ashby, Donal A. Walsh, Characterization of the Interaction of a Protein Inhibitor with Adenosine 3′,5′-Monophosphate-dependent Protein Kinases Journal of Biological Chemistry. ,vol. 247, pp. 6637- 6642 ,(1972) , 10.1016/S0021-9258(19)44739-X
K Weber, M Osborn, The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis Journal of Biological Chemistry. ,vol. 244, pp. 4406- 4412 ,(1969) , 10.1016/S0021-9258(18)94333-4
Erwin M. Reimann, Donal A. Walsh, Edwin G. Krebs, Purification and properties of rabbit skeletal muscle adenosine 3',5'-monophosphate-dependent protein kinases. Journal of Biological Chemistry. ,vol. 246, pp. 1986- 1995 ,(1971) , 10.1016/S0021-9258(19)77178-6
Max Costa, Endogenous protein kinase inhibitor levels regulate changes in specific activity of protein kinase in quiescent cells stimulated to proliferate. Biochemical and Biophysical Research Communications. ,vol. 78, pp. 1311- 1318 ,(1977) , 10.1016/0006-291X(77)91435-8
Jacques G. Demaille, Kristine A. Peters, Thomas P. Strandjord, Edmond H. Fischer, Isolation and properties of the bovine brain protein inhibitor of adenosine 3′:5′‐monophosphate‐dependent protein kinases FEBS Letters. ,vol. 86, pp. 113- 116 ,(1978) , 10.1016/0014-5793(78)80110-0