On line characterization of 58 phenolic compounds in Citrus fruit juices from Spanish cultivars by high-performance liquid chromatography with photodiode-array detection coupled to electrospray ionization triple quadrupole mass spectrometry.

摘要: Polyphenol profile of Citrus juices sweet orange, tangerine, lemon and grapefruit from Spanish cultivars was obtained by High-Performance Liquid Chromatography with Diode Array Detection coupled to Electrospray ionization Triple Quadrupole Mass Spectrometry. Fifty eight phenolic compounds five different classes were identified in these juices. Flavanone: O-dihexoside naringenin; flavones: apigenin-7-O-rutinoside-4'-O-glucoside, luteolin-7-O-neohesperidoside-4'-O-glucoside, luteolin-6-C-glucoside, 6,8-di-C-acylhexosides chrysoeriol diosmetin, 6C- 8C-glucoside-O-pentoside apigenin, apigenin-6-C-hexoside-O-hexoside apigenin-8-C-hexoside-O-acylrhamnoside; flavonols: 7-O-rutinosides quercetin, kaempferol, isorhamnetin tamarixetin, kaempferol-3-O-rutinoside, isorhamnetin-3-O-rutinoside-7-O-glucoside, tamarixetin-3-O-rutinoside-7-O-glucoside, isorhamnetin-3-O-hexoside-7-O-rhamnosylhexoside, 3-O-rhamnoside-7-O-rhamnosylhexoside quercetin kaempferol-3-O-rhamnosylhexoside-7-O-rhamnoside; hydroxycinnamic acids: O-hexoside ferulic sinapic acid; and, coumarins: O-rhamnosylhexoside scopoletin, had not previously been reported our knowledge. Structures have assigned on the basis complementary information retention time, UV-visible spectra, scan mode MS fragmentation patterns MS(2) spectra using collision energies. A structure diagnosis scheme is provided for identification compounds.