Efficient immobilization of phospholipase D on novel polymer supports with hierarchical pore structures

摘要: Abstract In this article, novel epoxy resin-based hierarchical porous polymers (HPSs) have been prepared through a non-sol-gel and template-free approach using crystalline trimethylolpropane (TMP) as porogen. The exhibit dimensional stability possess 3-dimentional interconnected multi-scale pores. range of 50 μm~10 nm are ultra-macro-pore in between skeleton, macro-pore on skeleton meso-pore network, respectively. porosity specific surface area can be adjusted 91.2–82.5% 225–156 m2/g, Using three kinds supports phospholipase D (PLD) was effectively immobilized physical adsorption. Owing to high the support improvement mass transfer loading amount PLD reached 223 mg/gsupport corresponding activity achieved up 3.75 × 103 U/gsupport. Under optimized conditions phosphatidylserine (PS) yield 95.5% within 40 min at 45 °C. exhibited not only better storage but also resistance pH thermal inactivation than free PLD. It found that 73.5% PS retained after 12 cycling reuses.