Stable transformation of barley via PEG-induced direct DNA uptake into protoplasts.
作者： P. A. Lazzeri , R. Brettschneider , R. Lührs , H. Lörz
DOI: 10.1007/BF00219433
关键词: Transformation (genetics) 、 Biology 、 Hordeum vulgare 、 Genetics 、 Southern blot 、 Genetic transfer 、 PEG ratio 、 Molecular biology 、 Protoplast 、 Callus 、 DNA
摘要: Protoplasts isolated from a barley cell suspension (cv Dissa) were transformed with plasmid DNA containing the neomycinphosphotransferase II (NPT) and β-glucuronidase (GUS) genes, using polyethyleneglycol (PEG) to induce uptake. Transformed microcalli selected in media G418 sulphate. NPT activity was detected all antibiotic-resistant lines, but not NPT-positive lines had GUS activity. Southern analysis confirmed presence of sequences homologous APT genes G418-resistant callus.
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