Transport-associated phosphorylation of 2-deoxy-D-glucose in Saccharomyces fragilis.
作者： H.T.A. Jaspers , J. van Steveninck
DOI: 10.1016/0005-2736(75)90017-6
关键词: Facilitated diffusion 、 Biochemistry 、 Metabolism 、 Biology 、 Substrate (chemistry) 、 2-Deoxy-D-glucose 、 Enzyme 、 Intracellular 、 Glucose transporter 、 Phosphorylation
摘要: Abstract 2-Deoxy- d -glucose transport and metabolism was studied in Saccharomyces fragilis . Inside the cells four phosphorylated three non-phosphorylated derivatives were found identified. Accumulation of 2-deoxyglucose balanced by a concomitant decrease cellular ATP, orthophosphate polyphosphates. The free sugar concentrated against concentration gradient, contradicting facilitated diffusion. Pulse labeling experiments revealed transport-associated phosphorylation. Theoretical considerations analysis effects iodoacetate showed that an intracellular hexokinase activity not involved phosphorylation, although this is good substrate for enzyme vitro experiments.
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