Aqueous Ocimum gratissimum extract induces cell apoptosis in human hepatocellular carcinoma cells.

摘要: Treatment of advanced hepatocellular carcinoma (HCC) has exhibited a poor overall survival rate only six to ten months, and the urgency development more effective novel agents is ever present. In this line research, we aimed investigate effects inhibitive mechanisms aqueous Ocimum gratissimum leaf extract (OGE), gratissimum, which commonly used as therapeutic herb for its numerous pharmacological properties, on malignant HCC cells. Our results showed that OGE decreased cell viability SK-Hep1 HA22T cells in dose-dependent manner (from 400 800 µg/mL), while there little effect Chang liver Moreover, cell-cycle analysis shows increased Sub-G1 count not observed These findings raise suspicion OGE-induced death may be mediated through proteins regulate cycle apoptosis cells, further experimentation revealed treatment resulted decrease caspase 3 PARP expressions CDK4and p-ERK1/2expressions. animal tests also tumor growth by treatment. We therefore suggest inhibition induced correlated alteration apoptosis-related proteins.