Efficient liver gene transfer with foamy virus vectors.
作者： Dimitris Zacharoulis , Chris Rountas , Michalis Katsimpoulas , John Morianos , Ilenia Chatziandreou
关键词: Virology 、 Virus 、 Polymerase chain reaction 、 Biology 、 Gene expression 、 Glyceraldehyde 3-phosphate dehydrogenase 、 Molecular biology 、 Gene delivery 、 Plasmid 、 Vector (molecular biology) 、 Real-time polymerase chain reaction
摘要: BACKGROUND Liver gene transfer offers hope for the correction of genetic and acquired disorders. Efficient in large animals can be obtained with hydrodynamic (HGT), a method that achieve sufficient levels delivery. MATERIAL AND METHODS To test relative efficiency between plasmid versus foamy virus (FV) vector-based liver efficiency, we applied HGT 4 juvenile pigs, using same backbone, either naked or coated as FV vector particle. Gene persistence expression was assayed by PCR real-time PCR, respectively, at 1 week month after infusions. RESULTS tolerated well no adverse reactions were observed. Plasmid injections resulted detectable DNA sequences week. At time point, 2/15 sections analyzed positive presence DNA. When vectors infused under identical conditions, 18/28 (64.3%) samples sequences, reached 29.7 15.6% endogenous GAPDH injected adjacent lobes. CONCLUSIONS Our results indicate medium-term therapeutic vectors, an effect attributed to potential procedure natural affinity hepatocytes.
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