Metabolic changes in Arabidopsis thaliana plants overexpressing chalcone synthase
作者： Thi Thanh Hien Dao
DOI:
关键词: Biology 、 Biochemistry 、 Gene 、 Metabolome 、 Metabolomics 、 Genetically modified crops 、 Botany 、 Transgene 、 Wild type 、 Chalcone synthase 、 Arabidopsis
摘要: The study has shown that it is possible to introduce the heterologous CHS gene in Arabidopsis thaliana and common multicopies of transgenes containing plants were obtained. Analysis change metabolome transgenic plants, high expression lines can be identified by markers such as flavonoids phenylpropanoids. It also clear UV-A/blue light stress does not further increase levels these marker compounds whereas wild type a treatment results increased compounds, fact similar plants. There are certain physiological limitations accumulation products. This thesis starts with review function especially plant resistance (Chapter 2). Chapter 3 deals work on Agrobacterium-mediated transformation chalcone synthase Col. 0. effect overexpression transcriptional level discribed this chapter. activity enzyme reported 4. In 5 metabolic profiling using nuclear magnetic resonance spectroscopy (NMR) described. chapter primary secondary metabolites 0 which detected NMR reported. 6 reports Arabidopsis. Metabolomic changes upon investigated 7). 8 non-pesticide chemical, Benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester (BTH) metabolome. Finally, general summary discussion given 9.
参考文章(275)
Werner Edgar Gläßgen, Anette Rose, Johannes Madlung, Wolfgang Koch, Johannes Gleitz, Hanns Ulrich Seitz, Regulation of enzymes involved in anthocyanin biosynthesis in carrot cell cultures in response to treatment with ultraviolet light and fungal elicitors Planta. ,vol. 204, pp. 490- 498 ,(1998) , 10.1007/S004250050283
P. Reymond, T. W. Short, W. R. Briggs, K. L. Poff, Light-induced phosphorylation of a membrane protein plays an early role in signal transduction for phototropism in Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 4718- 4721 ,(1992) , 10.1073/PNAS.89.10.4718
S. Tropf, T. Lanz, S.A. Rensing, J. Schr�der, G. Schr�der, Evidence that stilbene synthases have developed from chalcone synthases several times in the course of evolution. Journal of Molecular Evolution. ,vol. 38, pp. 610- 618 ,(1994) , 10.1007/BF00175881
M E Williams, R Foster, N H Chua, Sequences flanking the hexameric G-box core CACGTG affect the specificity of protein binding. The Plant Cell. ,vol. 4, pp. 485- 496 ,(1992) , 10.1105/TPC.4.4.485
Y. Xu, PFL. Chang, D. Liu, M. L. Narasimhan, K. G. Raghothama, P. M. Hasegawa, R. A. Bressan, Plant Defense Genes Are Synergistically Induced by Ethylene and Methyl Jasmonate. The Plant Cell. ,vol. 6, pp. 1077- 1085 ,(1994) , 10.1105/TPC.6.8.1077
Ouriel Faktor, Gary Loake, Richard A. Dixon, Chris J. Lamb, The G‐box and H‐box in a 39 bp region of a French bean chalcone synthase promoter constitute a tissue‐specific regulatory element Plant Journal. ,vol. 11, pp. 1105- 1113 ,(1997) , 10.1046/J.1365-313X.1997.11051105.X
E. SCHAFER, T. KUNKEL, H. FROHNMEYER, Signal transduction in the photocontrol of chalcone synthase gene expression Plant Cell and Environment. ,vol. 20, pp. 722- 727 ,(1997) , 10.1046/J.1365-3040.1997.D01-98.X
G. I. JENKINS, UV and blue light signal transduction in Arabidopsis Plant Cell and Environment. ,vol. 20, pp. 773- 778 ,(1997) , 10.1046/J.1365-3040.1997.D01-105.X
Joachim Schröder, A family of plant-specific polyketide synthases: facts and predictions Trends in Plant Science. ,vol. 2, pp. 373- 378 ,(1997) , 10.1016/S1360-1385(97)87121-X
Brenda Winkel-Shirley, Biosynthesis of flavonoids and effects of stress. Current Opinion in Plant Biology. ,vol. 5, pp. 218- 223 ,(2002) , 10.1016/S1369-5266(02)00256-X