Polyphosphonate induced coacervation of chitosan: encapsulation of proteins/enzymes and their biosensing.

摘要: Abstract Based on the polyphosphonate-assisted coacervation of chitosan, a simple and versatile procedure for encapsulation proteins/enzymes in chitosan–carbon nanotubes (CNTs) composites matrix was developed. Scanning electron microscopy (SEM), transmission (TEM), X-ray photoelectron spectroscopy (XPS), energy dispersive spectrum (EDS) mapping demonstrated hemoglobin (Hb) uniformly distributed into chitosan–CNTs matrix. Raman measurements indicated CNTs retained electronic structural integrities pristine CNTs. Fourier transform infrared (FT-IR), ultraviolet–visible (UV–vis) circular dichroism (CD) displayed encapsulated Hb preserved their near-native structure, indicating polyphosphonate–chitosan–CNTs possessed excellent biocompatibility proteins/enzymes. Electrochemical could directly exchange with substrate electrode. Moreover, modified electrode showed bioelectrocatalytic activity reduction hydrogen peroxide. Under optimum experimental conditions, fabricated electrochemical sensor fast response (less than 3 s), wide linear range (7.0 × 10−7 to 2.0 × 10−3 M) low detection limit (4.0 × 10−7 M) determination This newly developed protocol mild would certainly find extensive applications biocatalysis, biosensors, bioelectronics biofuel cells.