Multiple regions of Harvey sarcoma virus RNA can dimerize in vitro.
作者： Y X Feng , W Fu , A J Winter , J G Levin , A Rein
DOI: 10.1128/JVI.69.4.2486-2490.1995
关键词: Inverted repeat 、 Nucleic acid 、 Dimer 、 Nucleotide 、 Intron 、 Molecular biology 、 Stereochemistry 、 Nuclease protection assay 、 Biology 、 RNA 、 RNA editing
摘要: Retroviruses contain a dimeric RNA consisting of two identical molecules plus-strand genomic RNA. The structure the linkage between monomers is not known, but they are believed to be joined near their 5' ends. Darlix and coworkers have reported that transcripts retroviral sequences can dimerize spontaneously in vitro (see, for example, E. Bieth, C. Gabus, J. L. Darlix, Nucleic Acids Res. 18:119-127, 1990). As one approach identification which might participate linkage, we mapped derived from 378 bases Harvey sarcoma virus (HaSV) vitro. We found at least three distinct regions, nucleotides 37 229, 205 272, 271 378, form these dimers. Two regions 226; computer analysis suggests this region stem-loop with an inverted repeat loop. propose hypothetical involved dimer formation by transcripts. also compared thermal stabilities each dimers HaSV viral Dimers 229 272 both exhibited melting temperatures RNA, while quite unstable. formed degrees C less thermostable than same 55 It seems possible all present
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