Methods and compositions for enriching compositions for polymerase enzyme complexes
作者： Kamtekar Satwik , Miller Erik , Nyquist Kristofor , Bjornson Keith
DOI:
关键词: Biochemistry 、 Enzyme 、 Protease 、 Chemistry 、 Nucleic acid 、 Polymerase
摘要: The present invention provides methods, compositions, and systems for enriching compositions polymerase enzyme complexes. In particular, the of remove free polymerases from using one or more purification steps, including protease treatment, thus complexed with a template nucleic acid.
lens.org 参考文章(72)
Jonas Korlach, Stephen Turner, Modified base detection with nanopore sequencing ,(2013)
Mark A. Akeson, Gerald Maxwell Cherf, Michael Doody, Christopher Evan Lam, Kathy R. Lieberman, Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme ,(2011)
Keith Bjornson, Jeremiah Hanes, Stephen Turner, Real-time electronic sequencing ,(2014)
R L Novak, P Doty, The resistance of ribonucleic acid polymerase to proteolytic attack during transcription in vitro. Journal of Biological Chemistry. ,vol. 243, pp. 6068- 6070 ,(1968) , 10.1016/S0021-9258(18)94528-X
Takeshi Sano, Mark W. Pandori, Xiaomin Chen, Cassandra L. Smith, Charles R. Cantor, Recombinant Core Streptavidins A MINIMUM-SIZED CORE STREPTAVIDIN HAS ENHANCED STRUCTURAL STABILITY AND HIGHER ACCESSIBILITY TO BIOTINYLATED MACROMOLECULES Journal of Biological Chemistry. ,vol. 270, pp. 28204- 28209 ,(1995) , 10.1074/JBC.270.47.28204
Jonas Korlach, Hovig Bayandorian, Paul Lundquist, Cheng Frank Zhong, Mathieu Foquet, Substrates and optical systems and methods of use thereof ,(2009)
Jonas Korlach, Phospholink nucleotides for sequencing applications ,(2009)
Jonas Korlach, Jeffrey Wegener, Labeled reactants and their uses ,(2009)
Pezhman Monadgemi, Denis Zaccarin, Aaron Rulison, Paul Lundquist, Cheng Frank Zhong, Peiqian Zhao, Yanqiao Huang, Annette Grot, Ravi Saxena, Stephen Turner, High multiplex arrays and systems ,(2012)
Jonas Korlach, Keith Bjornson, Pranav Patel, Jeremiah Hanes, Arkadiusz Bibillo, Engineering polymerases and reaction conditions for modified incorporation properties ,(2009)