Combination effect of cetuximab with radiation in colorectal cancer cells

摘要: Aims and background. Colorectal cancer (CRC) is one of the commonest malignant disorders frequently associated with high expression epidermal growth factor receptor (EGFR), resulting in advanced disease a poor prognosis. In this study, we investigated radiosensitizing effects selective EGFR inhibitor cetuximab human CRC cell lines. Methods. Four lines, CaCo-2, HCT-8, LoVo, WiDr, were treated and/or radiation. The on proliferation viability measured by MTT annexin-V staining, clonogenic survival assay. vivo effect xenografts was assessed athymic nude mice. Results. Cetuximab combination radiation significantly inhibited vitro cells, concomitant increase death, except WiDr cells. Clonogenic assay confirmed that worked as radiosensitizer three cetuximab-sensitivie However, no correlations found between radiosensitivity level or mutation status signaling molecules. mice bearing xenografts, plus tumor over either agent alone. Interestingly, xenograft also sensitive to vivo, suggesting host-mediated cetuximab. Conclusions. enhanced cells efficiently growth. This study provided rationale for clinical application CRC. Free full text available at www.tumorionline.it.