In Vitro Testosterone–14C Metabolism by Rat Seminiferous Tubules at Different Stages of Development: Formation of 5α–Androstandiol at Meiosis

摘要: Whole testicular tissue, isolated seminiferous tubules and interstitial tissue of developing rats were incubated with testosterone–14C. After incubation, several steroid metabolites by paper thin—layer chromatography derivative formation. They usually accounted for more than 90% the radioactivity added as Losses corrected tritiated tracers. Identification products was confirmed recrystallization. 5α–Androstandiol– 14C main metabolite from tubules, dihydrotestosterone–14C androsterone– other minor products. 4 hr percentage conversion into 5α–androstandiol– at different ages follows: 1 day, 3.2; 10 days, 0.8; 11 0.3; 12 13 2.8; 14 15.7; 16 16.9; 18 34.4; 20 61.4; 45 62.5 365 32.6. In 20–day—old rats, much smaller, 12.4, in correspo...