Monitoring of chimerism after allogeneic bone marrow transplantation with unmanipulated marrow by use of DNA polymorphisms.

摘要: Highly polymorphic tandemly repetitive DNA sequences provide powerful genetic markers for the identification of individuals by restriction fragment length polymorphisms (RFLP) even in close relatives. Over a three-year period, 61 consecutive patients from single institution undergoing allogeneic bone marrow transplantation (BMT) various hematological diseases were grafted with unmanipulated and followed development hematopoietic chimerism. Three synthetic oligonucleotide probes homologous to so-called minisatellite or variable number tandem repeat (VNTR) evaluated clinical setting BMT their usefulness: (i) document engraftment rejection; (ii) elucidate kinetics mixed chimerism; (iii) providing sensitive tool early detection relapse. In addition, CML karyotyping analysis bcr/abl gene rearrangement was performed. Using this panel three probes, informative specific donor recipient RFLP could be demonstrated all cases. Engraftment documented surviving beyond day +14 after BMT. Mixed chimerism detected 14% phase (day +78) but only one patient turned out become long-term stable chimera. These results support hypothesis that lymphocytes origin conditioning regimen may considerably contribute Long-term is rare event marrow. Simultaneous VNTR-RFLP, karyotyping, showed corresponding nine 12 patients. either methods failed detect residual cells Therefore different assessment seem complement rather than exclude each other. Eleven who exhibited complete relapsed underlying disease. seven these acute leukemia, DNA-RFLP had been performed shortly before relapse (30-86 days) herald As sensitivity minor cell population DNA-RFLPs approximately 1%, data indicate leukemia characterized sudden increase percentage blast not detectable frequent analyses.