BIOCHEMICAL STUDY OF ENDONUCLEASE V AND ITS APPLICATION IN MUTATION SCANNING
作者： Honghai Gao
DOI:
关键词: Biology 、 Endonuclease 、 DNA repair 、 Molecular biology 、 Premature aging 、 DNA 、 Mutation 、 Mutant 、 DNA deamination 、 DNA damage
摘要: The integrity of the genetic information encoded by DNA is essential to all living organisms, yet reactive bases are constantly attacked endogenous and exogenous agents resulting in as many one million individual molecular lesions per cell day. Excessive damage or deficiency repair enzymes may cause cancer, premature aging, neurodegenerative diseases. Endonuclease V (Endo V) a enzyme which can recognize four types deamination products, specifically, uracil, hypoxanthine, xanthine oxanine. It was also shown that endo mismatches. We screened about 60 mutants from Thermotoga maritima found some had altered base preferences for Tma Y80A become C-specific mismatch endonuclease. G13D mutation K-ras oncogene not recognized wild type successfully cleaved Y80A. This study provides valuable on recognition active site organization V. be used cancer scanning recognition. In order further understand role Y80 recognition, we substituted with sixteen amino acids. Together three isolated before, characterized nineteen using deaminated basecontaining substrates mismatch-containing substrates. comprehensive acid substitution at single (Y80) underlines importance aromatic ring hydrogen bond donor capacity V, reveals
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