Regulation of expression of the wound tumor virus genome in persistently infected vector cells is related to change in translational activity of viral transcripts.

摘要: The interaction between a plant virus and its insect vector was studied at the molecular level by examining wound tumor (WTV) gene expression in cultured cells derived from leafhopper vector. Infection of WTV is noncytopathic results an acute phase (through day 5), followed persistence beginning with first cell passage. Viral-specific polypeptide synthesis viral genome RNA accumulation increased to maximum during 5 days following inoculation then decreased as infected were passaged (to 20% observed passages 10 15). In contrast, viral-specific mRNAs present approximately same early stage (passage 10) persistent infection. Although transcripts isolated different times after exhibited identical electrophoretic migration patterns, they had functional activities cell-free translation systems. Transcripts persistently inefficiently translated vitro, reflecting situation cells. These indicate that decline associated infection related change translational activity transcripts. Images