Androgen synthesis and aromatization by equine corpus luteum microsomes.

摘要: Whereas mare corpus luteum does not produce androgens or estrogens in vivo, the incubation of microsomes with progesterone and NADPH resulted 17 alpha-hydroxyprogesterone estrogen production a small yield androstenedione. In presence an aromatase inhibitor (4-hydroxyandrostenedione), androstenedione were accumulated. Aromatization testosterone occurred via stereospecific loss 1 beta, 2 beta hydrogen atoms was inhibited by MgCl2, KCl, EDTA. The Km synthetase from equine for 18.5 +/- 2.7 nM 11.5 1.5 nM. 19-Norandrogens aromatized slightly higher efficiency than androgens, but affinity lower 19-norandrogens androgens. Our results suggest that aromatases testis are closely related enzymes. On other hand, question arises as to relationship among cell origin, synthetizing abilities, vivo different mammalian species.