[Comparison of different methods of molecular-genetic analysis of somatic mutations in K-ras gene in patients with colorectal cancer].
作者： F. A. Amosenko , I. V. Karpov , A. V. Polyakov , S. P. Kovalenko , V. A. Shamanin
关键词: Genetics 、 Molecular biology 、 In patient 、 Point mutation 、 Colorectal cancer 、 Biology 、 genomic DNA 、 Gene 、 Single-strand conformation polymorphism 、 Somatic cell 、 Molecular analysis
摘要: Two approaches to somatic point mutations in 12 and 13 codones of K-ras gene were analyzed: PCR/SSCP/AСRS/sequencing allele-specific PCR the real-life regimen (Russian set «KRAS-7M»). The comparison was carried out on 62 examples genomic DNA extracted from frozen colon carcinomas, which underwent manual dissection. results obtained two attempts consistent 95,2% (N=59). Specificity sensitivity detection using «KRAS-7M» 100 96,4% respectively, 94,1 100% respectievly PCR/SSCP/AСRS/ automatic sequencing. False positive absent when detecting with accounted for 2 cases (5,9%) PCR/SSCP/ AСRS/automatic only false negative response (3,6%) analyzing «KRAS-7M».
sci-hub.st 参考文章(38)
T Minamoto, Z Ronai, M Mai, K-ras mutation: early detection in molecular diagnosis and risk assessment of colorectal, pancreas, and lung cancers--a review. Cancer Detection and Prevention. ,vol. 24, pp. 1- 12 ,(2000)
M. Ronaghi, DNA SEQUENCING:A Sequencing Method Based on Real-Time Pyrophosphate Science. ,vol. 281, pp. 363- 365 ,(1998) , 10.1126/SCIENCE.281.5375.363
Bret Taback, Anton J. Bilchik, Sukamal Saha, Takahiro Nakayama, David A. Wiese, Roderick R. Turner, Christine T. Kuo, Dave S. B. Hoon, Peptide nucleic acid clamp PCR: a novel K-ras mutation detection assay for colorectal cancer micrometastases in lymph nodes. International Journal of Cancer. ,vol. 111, pp. 409- 414 ,(2004) , 10.1002/IJC.20268
Matilde E Lleonart, Santiago Ramón y Cajal, John D Groopman, Marlin D Friesen, Sensitive and specific detection of K‐ras mutations in colon tumors by short oligonucleotide mass analysis Nucleic Acids Research. ,vol. 32, ,(2004) , 10.1093/NAR/GNH051
Niels Smakman, Inne H.M. Borel Rinkes, Emile E. Voest, Onno Kranenburg, Control of colorectal metastasis formation by K-Ras Biochimica et Biophysica Acta. ,vol. 1756, pp. 103- 114 ,(2005) , 10.1016/J.BBCAN.2005.07.001
Cara C. Tigue, Karen A. Fitzner, Motasem Alkhatib, Eric Schmid, Charles L. Bennett, The value of innovation : the economics of targeted drugs for cancer Targeted Oncology. ,vol. 2, pp. 113- 119 ,(2007) , 10.1007/S11523-007-0043-8
Jens Bjørheim, Sigrid Lystad, Annika Lindblom, Ulf Kressner, Sophia Westring, Siobhan Wahlberg, Gudrun Lindmark, Gustav Gaudernack, Per Ekstrøm, Janne Røe, William G. Thilly, Anne-Lise Børresen-Dale, Mutation analyses of KRAS exon 1 comparing three different techniques: temporal temperature gradient electrophoresis, constant denaturant capillary electrophoresis and allele specific polymerase chain reaction. Mutation Research. ,vol. 403, pp. 103- 112 ,(1998) , 10.1016/S0027-5107(98)00057-8
Shuji Ogino, Takako Kawasaki, Mohan Brahmandam, Liying Yan, Mami Cantor, Chungdak Namgyal, Mari Mino-Kenudson, Gregory Y. Lauwers, Massimo Loda, Charles S. Fuchs, Sensitive Sequencing Method for KRAS Mutation Detection by Pyrosequencing The Journal of Molecular Diagnostics. ,vol. 7, pp. 413- 421 ,(2005) , 10.1016/S1525-1578(10)60571-5
Angela Poehlmann, Doerthe Kuester, Frank Meyer, Hans Lippert, Albert Roessner, Regine Schneider-Stock, K-ras mutation detection in colorectal cancer using the Pyrosequencing technique. Pathology Research and Practice. ,vol. 203, pp. 489- 497 ,(2007) , 10.1016/J.PRP.2007.06.001
Irena Porębska, Antonina Harłozińska, Tomasz Bojarowski, Expression of the tyrosine kinase activity growth factor receptors (EGFR, ERB B2, ERB B3) in colorectal adenocarcinomas and adenomas. Tumor Biology. ,vol. 21, pp. 105- 115 ,(2000) , 10.1159/000030116