HEMOPOIETIC COLONY STUDIES V. EFFECT OF HEMOPOIETIC ORGAN STROMA ON DIFFERENTIATION OF PLURIPOTENT STEM CELLS

摘要: In heavily irradiated mice, bone marrow regeneration of either endogenous or exogenous origin was shown to occur in discrete foci comparable the more intensively studied spleen colonies. The number colonies inversely related dose whole body X-irradiation. Endogenous were found after higher doses irradiation than Most them granulocytic nature. Exogenous lethally mice injected with cells proportional injected, appeared at a time like which, 7 8 days, they single differentiated cell line, granuloid erythroid megakaryocytic, small percentage "mixed" Whereas outnumber spleen, situ subcutaneously transplanted (E:G colony ratio about 3.5), 0.7). characteristic E:G ratios and appear likely be result hemopoietic organ stromal influence on pluripotent forming units (CFU's) selective lodgment committed (unipotent) CFU's respectively, as indicated by following. Bone stem (CFU) which had reseeded cavity primary recipients 18–24 hr earlier, reharvested retransplanted intravenously into secondary hosts. formed hosts typical (2.8), that (0.6). Pieces stroma containing CPU's from contralateral femur these same implanted trocar directly spleens other recipients. Those developed intrasplenic-implanted yielded an. 0.1. migrated contiguous remote portions gave 2.9 2.4, respectively. Irradiated normal (a 1 mm cube spleen) loaded mice. five two mixed larger host an higher. Of those 19 bridged junction each above experiments, every case, portion splenic stroma, stroma.