Ultrahigh-pressure reversed-phase liquid chromatography in packed capillary columns.

摘要: The use of extremely high pressures in liquid chromatography can improve the efficiency and reduce analysis time for columns packed with small particles. In this work, fused-silica capillaries inner diameters 30 μm are slurry 1.5 nonporous octadecylsilane-modified silica These prepared lengths up to 66 cm packing as 4100 bar (60 000 psi). Near optimum flow rate, generate many 300 000 theoretical plates lightly retained compounds (k‘ < 0.5) over 200 000 more ≈ 2). translate plate heights (Hmin) low 2.1 μm. required run at rates on order 1400 (20 000 Analysis times these min 2) be reduced less than 10 higher rates. Capacity factors observed increase linearly applied pressure.