New Types of Toxin A-Negative, Toxin B-Positive Strains among Clostridium difficile Isolates from Asia

摘要: A total of 56 C. difficile strains were selected from 310 isolates obtained different hospitals in Japan and Korea healthy infants Indonesia. Strains that had been previously typed by pulsed-field gel electrophoresis PCR ribotyping, characterized toxinotyping binary toxin gene detection. When toxinotyped, 35 determined to be toxinotype 0, whereas 21 showed variations genes could grouped into 11 variant toxinotypes. Six the toxinotypes described before (I, III, IV, VIII, IX, XII). In addition, five new defined (XVI XX). Three (XVIII, XIX, XX) vary only repetitive regions tcdA produce both toxins. two XVI XVII, production TcdA not detected with commercial immunological kits. Strain J9965 (toxinotype XVII) was PaLoc similar but identical another known A−B+ strain, 8864. SUC 36 XVI), on other hand, well-defined group consisting V, VI, VII, which thus far includes A+B+ strains. Toxinotypes XVII represent groups well-known VIII have a nonsense mutation at beginning gene, introduction stop codon amino acid position 47 results nonproduction TcdA. The 5′-end sequence newly does contain an mutation. prevalence varied greatly among nine hospitals. Only four positive for amplification gene.