P-0269 Circulating Endothelial Cells in Patients with Colorectal Cancer: Flow Cytometry Analysis of Cell Markers CD146-PE and CD45-FITC

摘要: ABSTRACT Introduction Colorectal cancer (CRC) remains the third most common both in men and women worldwide, its incidence rates continue to increase USA Western Europe. Angiogenesis is crucial for growth of all tumors, mature immature endothelial cells, such as circulating cells (CECs) progenitor are present human blood. CECs extremely rare normal peripheral blood, but it has been shown that their number significantly increased many pathological conditions, including cancer. Some Among CECs display characteristics terminally differentiated while others express specific surface antigens, suggesting a progenitor-like or stem-like phenotype. Thus, vasculogenesis not restricted embryonic development, there putative progenitors which can participate generation new vessels. measurement promising biomarker assess efficacy antiangiogenic therapies, may suggest possible presence metastatic disease, patients with CRC. Different cell markers have tested detect CECs, flow cytometry represents well suited method detection quantitation. The aim this study was evaluate levels microenvironmental marker CD146+CD45- phenotype, linked angiogenesis, vessel damage, disease progression, pT0-1 Methods Blood samples from 41 (26 men, 15 women, median age 64 years, range 39-74 years) confirmed colorectal adenocarcinoma undergoing curative surgery were collected analysis CECs. Patients distant metastases (negative F-18 FDG PET/CT) excluded, those who had undergone adjuvant chemotherapy. Written informed consent obtained participants. All stained antihuman CD146 phycoerythrin (CD146-PE) CD45 fluorescein isothiocyanate (CD45-FITC). Immunophenotyping using Beckman Coulter XL-MCL cytometer 600 s acquisition time each sample. According to final pathology, two groups obtained: Group A (28 patients, 68.3%) negative lymph nodes (pN0), B (13 31.7%) regional node metastasis (pN1). Results N1 (Group B) level CD146+/CD45- (19.2±9.1 vs. 12.4±8.6 95% CI 0.72-12.88, p=0.029) respect N0 B). No correlation (R=0.10, p=0.22) found between There no difference (p=NS) women. Conclusion Although population (less than one 1000 blood cells) yet completely established phenotype represent technical challenge, our preliminary data angiogenic activity CRC, be demonstrated Further markers, alone combination, should studied confirm hypothesis.