Prevalence and numbers of Escherichia coli O157 on bovine hides at a beef slaughter plant.
作者： S. B. O[apos]BRIEN , G. DUFFY , E. CARNEY , J. J. SHERIDAN , D. A. McDOWELL
DOI: 10.4315/0362-028X-68.4.660
关键词: Novobiocin 、 Immunomagnetic separation 、 Microbiology 、 Sorbitol 、 Veterinary medicine 、 Pcr analysis 、 Biology 、 Cefixime 、 Escherichia coli 、 Rump 、 Agar
摘要: In this study, we investigated the prevalence and numbers of Escherichia coli O157 on bovine hides. Samples (n = 1,500) were collected over a 17-month period (30 samples per week) by sponge swabbing approximately 122-cm2 areas rump slaughtered cattle at an early stage carcass processing (first legging). Sponge stomached in buffered peptone water supplemented with novobiocin, directly plated sorbitol MacConkey Cefixime tellurite (SMAC-CT), enriched for 24 h, extracted immunomagnetic separation, onto SMAC-CT agar. Presumptive E. colonies from plates confirmed PCR presence eaeA, hlyA, fliCh7, vt1, vt2, portions rfb (O-antigen encoding) region O157. Overall, was recovered 109 (7.3%) concentrations ranging less than 0.13 to 4.24 log CFU/100 cm2. analysis revealed wide diversity genetic profiles among isolates verocytotoxigenic c...
allenpress.com
unirioja.es
jfoodprotection.com参考文章(29)
WARREN J. DORSA, CATHERINE N. CUTTER, GREGORY R. SIRAGUSA, MOHAMMAD KOOHMARAIE, Microbial Decontamination of Beef and Sheep Carcasses by Steam, Hot Water Spray Washes, and a Steam-Vacuum Sanitizer. Journal of Food Protection. ,vol. 59, pp. 127- 135 ,(1996) , 10.4315/0362-028X-59.2.127
MICHAEL H. CASSIN, GREG M. PAOLI, ANNA M. LAMMERDING, Simulation modeling for microbial risk assessment. Journal of Food Protection. ,vol. 61, pp. 1560- 1566 ,(1998) , 10.4315/0362-028X-61.11.1560
J. R. RANSOM, K. E. BELK, R. T. BACON, J. N. SOFOS, J. A. SCANGA, G. C. SMITH, Comparison of sampling methods for microbiological testing of beef animal rectal/colonal feces, hides, and carcasses. Journal of Food Protection. ,vol. 65, pp. 621- 626 ,(2002) , 10.4315/0362-028X-65.4.621
J. M. McEVOY, A. M. DOHERTY, J. J. SHERIDAN, I. S. BLAIR, D. A. McDOWELL, Use of Steam Condensing at Subatmospheric Pressures To Reduce Escherichia coli O157:H7 Numbers on Bovine Hide Journal of Food Protection. ,vol. 64, pp. 1655- 1660 ,(2001) , 10.4315/0362-028X-64.11.1655
PINA M. FRATAMICO, LORI K. BAGI, TIZIANA PEPE, A multiplex polymerase chain reaction assay for rapid detection and identification of Escherichia coli O157:H7 in foods and bovine feces. Journal of Food Protection. ,vol. 63, pp. 1032- 1037 ,(2000) , 10.4315/0362-028X-63.8.1032
RANDALL K. PHEBUS, ABBEY L. NUTSCH, DAVID E. SCHAFER, R. CRAIG WILSON, M. JAMES RIEMANN, JEROME D. LEISING, CURTIS L. KASTNER, JOHN R. WOLF, RAM K. PRASAI, Comparison of Steam Pasteurization and Other Methods for Reduction of Pathogens on Surfaces of Freshly Slaughtered Beef. Journal of Food Protection. ,vol. 60, pp. 476- 484 ,(1997) , 10.4315/0362-028X-60.5.476
ABBEY L. NUTSCH, RANDALL K. PHEBUS, M. JAMES RIEMANN, JOHN S. KOTROLA, R. CRAIG WILSON, JOHN E. BOYER, TED L. BROWN, Steam Pasteurization of Commercially Slaughtered Beef Carcasses: Evaluation of Bacterial Populations at Five Anatomical Locations Journal of Food Protection. ,vol. 61, pp. 571- 577 ,(1998) , 10.4315/0362-028X-61.5.571
Van Donkersgoed J, Graham T, Gannon, The prevalence of verotoxins, Escherichia coli O157:H7, and Salmonella in the feces and rumen of cattle at processing. Canadian Veterinary Journal-revue Veterinaire Canadienne. ,vol. 40, pp. 332- 338 ,(1999)
ABBEY L. NUTSCH, RANDALL K. PHEBUS, M. JAMES RIEMANN, DAVID E. SCHAFER, J. E. BOYER, R. CRAIG WILSON, JEROME D. LEISING, CURTIS L. KASTNER, Evaluation of a Steam Pasteurization Process in a Commercial Beef Processing Facility Journal of Food Protection. ,vol. 60, pp. 485- 492 ,(1997) , 10.4315/0362-028X-60.5.485
Adrienne W. Paton, James C. Paton, Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157 Journal of Clinical Microbiology. ,vol. 36, pp. 598- 602 ,(1998) , 10.1128/JCM.36.2.598-602.1998