Characterization of HOX gene expression during myelopoiesis: role of HOX A5 in lineage commitment and maturation.

摘要: During the process of normal hematopoiesis, proliferation is tightly linked to maturation. The molecular mechanisms that lead production mature effector cells with a variety phenotypes and functions from single multipotent progenitor are only beginning be elucidated. It important determine how these maturation events regulated at level, because this will provide significant insights into hematopoiesis as well leukemogenesis. Transcription factors containing highly conserved homeobox motif show considerable promise potential regulators hematopoietic events. In study, we focused on identification characterization genes HOX family in regulating human myeloid differentiation induced by growth factor, granulocyte-macrophage colony-stimulating factor (GM-CSF). We have identified three genes, A5, B6, B7, which expressed during early myelopoiesis. Treating bone marrow antisense oligodeoxynucleotides A5 resulted inhibition granulocytic/monocytic increased generation erythroid progenitors. Also, overexpression inhibited K562 cell line. Based observations, propose an regulator lineage determination