Fluorescence correlation microscopy (FCM)-fluorescence correlation spectroscopy (FCS) taken into the cell.

摘要: Confocal fluorescence correlation spectroscopy (FCS) and other confocal spectroscopic techniques are ideally suited for the analysis of molecular interactions at subcellular level. However, one requires exact positioning in three dimensions within cell. Our instrument integrates FCS with high sensitivity digital imaging microscopy precision positioning. We present first measurements intracellular FCS, specification instrumental requirements methods data analysis. propose term (FCM) this extended modality FCS.