Shear Stress Increases the Residence Time of Adhesion of Pseudomonas aeruginosa

摘要: Although ubiquitous, the processes by which bacteria colonize surfaces remain poorly understood. Here we report results for influence of wall shear stress on early-stage adhesion Pseudomonas aeruginosa PA14 glass and polydimethylsiloxane surfaces. We use image analysis to measure residence time each adhering bacterium under flow. Our main finding is that, either surface, characteristic increases approximately linearly as (∼0–3.5 Pa). To investigate this phenomenon, used mutant strains defective in surface organelles (type I pili, type IV or flagellum) extracellular matrix production. show although these bacterial features frequency events detachment probability, none them responsible trend shear-enhanced time. These observations bring what believe are new insights into mechanism attachment flows, suggest a role other intrinsic cell dynamic response stress.