作者: Joseph R. Lakowicz
DOI: 10.1007/978-1-4757-3061-6_14
关键词: Measure (mathematics) 、 Emission spectrum 、 Energy transfer 、 Acceptor 、 Absorption spectroscopy 、 Resonance (particle physics) 、 Physics 、 Förster resonance energy transfer 、 Molecular physics 、 Macromolecule
摘要: In the previous chapter we described principles of resonance energy transfer and how phenomenon could be used as a “spectroscopic ruler” to measure distances between donor acceptor sites on macromolecules. Energy was through-space interaction which occurred whenever emission spectrum overlapped with absorption acceptor. For given donor—acceptor (D—A) pair, efficiency decreases r −6, where is D—A distance. Each pair has characteristic distance, Forster distance (R 0), at RET 50% efficient. The extent transfer, seen from steady-state data, can determine association based proximity, or closest approach pair.