Time-Resolved Energy Transfer and Conformational Distributions of Biopolymers

作者: Joseph R. Lakowicz

DOI: 10.1007/978-1-4757-3061-6_14

关键词: Measure (mathematics)Emission spectrumEnergy transferAcceptorAbsorption spectroscopyResonance (particle physics)PhysicsFörster resonance energy transferMolecular physicsMacromolecule

摘要: In the previous chapter we described principles of resonance energy transfer and how phenomenon could be used as a “spectroscopic ruler” to measure distances between donor acceptor sites on macromolecules. Energy was through-space interaction which occurred whenever emission spectrum overlapped with absorption acceptor. For given donor—acceptor (D—A) pair, efficiency decreases r −6, where is D—A distance. Each pair has characteristic distance, Forster distance (R 0), at RET 50% efficient. The extent transfer, seen from steady-state data, can determine association based proximity, or closest approach pair.

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