Cell permeabilization and inhibition of voltage-gated Ca2+ and Na+ channel currents by nanosecond pulsed electric field
作者： Vasyl Nesin , Angela M. Bowman , Shu Xiao , Andrei G. Pakhomov
DOI: 10.1002/BEM.21696
关键词: Potassium channel 、 Nuclear magnetic resonance 、 Nanosecond 、 Electroporation 、 Voltage-dependent calcium channel 、 Sodium channel 、 Voltage-gated ion channel 、 Cell membrane 、 Chemistry 、 Patch clamp
摘要: Previous studies have found that nanosecond pulsed electric field (nsPEF) exposure causes long-term permeabilization of the cell plasma membrane. In this study, we utilized whole-cell patch-clamp method to study nsPEF effect on currents voltage-gated (VG) Ca(2+) and Na(+) channels (I(Ca) I(Na)) in cultured GH3 NG108 cells. We a single 300 or 600 ns pulse at above 1.5-2 kV/cm caused prolonged inhibition I(Ca) I(Na). Concurrently, increased non-inactivating "leak" current (I(leak)), presumably due formation nanoelectropores larger pores The effects were similar cells exposed intact subsequently brought into recording configuration, first configuration then exposed. Although both I(leak) VG enhanced higher E-field levels, these two showed relatively weak correlation with each other. some cells, 10-fold more while remained unchanged. At longer time intervals after (5-15 min), I(Na) could remain inhibited although had largely recovered. causal relation inhibitory membrane is discussed.
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