Development and evaluation of a rapid enzyme-immunoassay system for measurement of the urinary concentration of estrone-3-glucuronide in a female giant panda (Ailuropoda melanoleuca).
作者： Natsuki HAMA , Hideyasu KANEMITSU , Kensuke SAKAMOTO , Yujiro OYAMA , Tomas J. ACOSTA
DOI: 10.1262/JRD.19166
关键词: Ailuropoda melanoleuca 、 Estrous cycle 、 Endocrinology 、 Immunoassay 、 Biology 、 Ailuropoda 、 Radioimmunoassay 、 Internal medicine 、 Estrone 、 Chromatography 、 Urinary system 、 Urine
摘要: To detect estrus for reproductive management, and to determine the relationship between urinary estrogen estrous behavior, in a female giant panda, we developed evaluated rapid enzyme immunoassay (EIA) system Estrone-3-glucuronide (E1G) using commercial reagents. The EIA took only around 3 hours, including all procedures obtain result. It indicated good reproducibility (intra-assay CV of 5.16%, interassay 15.4%) sensitivity (lowest standard concentration was 0.0156 ng/ml) measurement concentrations E1G panda. There positive correlation (r=0.934) with data estrone (E1) same samples, as measured by radioimmunoassay (RIA) performed laboratory. changes were almost synchronous E1 assayed RIA urine collected during 4 consecutive seasons. dynamics this highly correlated occurrence presenting behavior above results indicate that assay may be normally, rapidly practically used
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