Novel haloalkaliphilic methanotrophic bacteria: An attempt for enhancing methane bio-refinery.

摘要: Abstract Methane bioconversion into products with a high market value, such as ectoine or hydroxyectoine, can be optimized via isolation of more efficient novel methanotrophic bacteria. The research here presented focused on the enrichment consortia able to co-produce different ectoines during CH4 metabolism. Four enrichments (Cow3, Slu3, Cow6 and Slu6) were carried out in basal media supplemented 3 6% NaCl, using methane sole carbon energy source. highest accumulation (∼20 mg g biomass−1) was recorded two enriched at NaCl (Cow6 Slu6). Moreover, hydroxyectoine detected for first time feedstock Slu6 (∼5 mg g biomass−1). majority haloalkaliphilic bacteria identified by 16S rRNA community profiling both have not been previously described methanotrophs. From these enrichments, strains (representing species) capable source isolated: Alishewanella sp. strain RM1 Halomonas PGE1. PGE1 showed higher yields (70–92 mg than those other methanotrophs under continuous cultivation mode (∼37–70 mg results obtained highlight potential isolating order boost competitiveness industrial CH4-based production.