Effect of curcumin (Curcuma longa extract) on LPS-induced acute lung injury is mediated by the activation of AMPK

摘要: Curcumin, a biphenolic compound extracted from turmeric (Curcuma longa), possesses potent anti-inflammatory activity. The present study investigated whether curcumin could increase 5′ adenosine monophosphate-activated protein kinase (AMPK) activity in macrophages and modulate the severity of lipopolysaccharide (LPS)-induced acute lung injury. Macrophages were treated with then exposed (or not) to LPS. Acute injury was induced by intratracheal administration LPS BALB/c mice. Curcumin increased phosphorylation AMPK acetyl-CoA carboxylase (ACC), downstream target AMPK, time- concentration-dependent manner. did not liver B1, primary upstream AMPK. STO-609, an inhibitor calcium2+/calmodulin-dependent kinase, diminished curcumin-induced phosphorylation, but transforming growth factor-beta-activated 1 not. also LPS-induced inhibitory κB-alpha production tumor necrosis factor alpha (TNF-α), macrophage inflammatory (MIP)-2, interleukin (IL)-6 macrophages. Systemic significantly decreased TNF-α, MIP-2, IL-6 as well neutrophil accumulation bronchoalveolar lavage fluid, pulmonary myeloperoxidase levels wet/dry weight ratio mice subjected treatment. These results suggest that protective effect on is associated activation.