Construction of a high-performance human fetal liver-derived lentiviral cDNA library

摘要: The gene transduction method is a very powerful tool, not only in basic science but also clinical medicine. Regenerative medicine one field that has close connection with both and clinical. Recently, it been reported induced pluripotent stem (iPS) cells can be produced from somatic by three or four transduction. We have recently lentiviral transfer of the tal1/scl efficiently differentiate non-human primate common marmoset ES into hematopoietic without support stromal cells. In this study, we constructed high-performance human fetal liver-derived expression library, which contains high number individual clones, order to develop helpful tool for understanding early hematopoiesis and/or hepatocytosis future regenerative Our cDNA library consisted more than 8 × 107 their average insert size was >2 kb. DNA sequence analysis each inserted cDNAs revealed >60% contained full-length protein-coding regions many genes including cytokine receptors, cytoplasmic proteins, protein inhibitors, nuclear factors. efficiency on 293T 100% an integrated ~1.1 These results suggest our liver could accelerating discovery novel are involved hepatopoiesis make use iPS efficient