Bile Acid Aspiration Associated With Lung Chemical Profile Linked to Other Biomarkers of Injury After Lung Transplantation

摘要: Aspiration of gastrointestinal contents has been linked to worse outcomes following lung transplantation but uncertainty exists about underlying mechanisms. We applied high-resolution metabolomics bronchoalveolar lavage fluid (BALF) in patients with episodic aspiration (defined by bile acids the BALF) identify potential metabolic changes associated aspiration. Paired samples, one and another without, from 29 stable transplant were studied. Liquid chromatography coupled mass spectroscopy was used interrogate metabolomic these samples. Data obtained for 7068 ions representing intermediary metabolites, environmental agents chemicals microbial colonization. A substantial number (2302) differed between acid positive negative samples when analyzed false discovery rate at q = 0.01. These included pathways metabolism. Hierarchical cluster analysis defined clusters that correlated previously reported biomarkers injury including T cell granzyme B level chemoattractants CXCL9 CXCL10. data specifically link presence allografts inflammatory known segregate worsening allograft outcome, provide additional mechanistic insight into association reflux injury.