Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity

作者: A. Nostro , M.P. Germano , V. D'Angelo , A. Marino , M.A. Cannatelli

DOI: 10.1046/J.1472-765X.2000.00731.X

关键词: TerpeneFlavonoidTerpenoidAntimicrobialMinimum inhibitory concentrationBiologyMedicinal plantsPhytochemicalTraditional medicineMicrobiologyGram-positive bacteria

摘要: A comparative study on the antimicrobial properties of extracts from medicinal plants obtained by two different methods was carried out. The screening activity six conducted a disc diffusion test against Gram-positive, -negative and fungal organisms. most active (inhibition diameter ≥12 mm) were assayed for minimum inhibitory concentration submitted to phytochemical thin-layer chromatography bioautography. results indicate that diethyl ether efficient compounds. more pronounced Gram-positive organisms than Gram-negative bacteria. Bioautography showed probably due flavonoids terpenes.

参考文章(17)
R FACINO, M CARINI, L FRANZOI, O PIROLA, E BOSISIO, Phytochemical characterization and radical scavenger activity of flavonoids from Helichrysum italicum G. Don (compositae) Pharmacological Research. ,vol. 22, pp. 709- 721 ,(1990) , 10.1016/S1043-6618(05)80097-0
J. B. Harborne, Methods of Plant Analysis Springer, Dordrecht. pp. 1- 36 ,(1984) , 10.1007/978-94-009-5570-7_1
C. Bourrel, F. Perineau, G. Michel, J. M. Bessiere, Catnip (Nepeta cataria L.) essential oil: analysis of chemical constituents, bacteriostatic and fungistatic properties Journal of Essential Oil Research. ,vol. 5, pp. 159- 167 ,(1993) , 10.1080/10412905.1993.9698195
Rene Scherrer, Philipp Gerhardt, Molecular Sieving by the Bacillus megaterium Cell Wall and Protoplast Journal of Bacteriology. ,vol. 107, pp. 718- 735 ,(1971) , 10.1128/JB.107.3.718-735.1971
K. A. Hammer, C. F. Carson, T. V. Riley, Antimicrobial activity of essential oils and other plant extracts Journal of Applied Microbiology. ,vol. 86, pp. 985- 990 ,(1999) , 10.1046/J.1365-2672.1999.00780.X
T. Mezzetti, G. Orzalesi, C. Rossi, V. Bellavita, A new triterpenoid lactone, alpha-amyrin and uvaol from Helichrysum italicum. Planta Medica. ,vol. 18, pp. 326- 331 ,(1970) , 10.1055/S-0028-1099786
B. A. Lajubutu, R. J. Pinney, M. F. Roberts, H. A. Odelola, B. A. Oso, Antibacterial activity of diosquinone and plumbagin from the root of Diospyros mespiliformis (Hostch) (Ebenaceae) Phytotherapy Research. ,vol. 9, pp. 346- 350 ,(1995) , 10.1002/PTR.2650090508
M. Lis-Balchin, S.G. Deans, Antimicrobial effects of hydrophilic extracts of Pelargonium species (Geraniaceae) Letters in Applied Microbiology. ,vol. 23, pp. 205- 207 ,(1996) , 10.1111/J.1472-765X.1996.TB00066.X
C. Bourrel, G. Vilarem, F. Perineau, Chemical Analysis, Bacteriostatic and Fungistatic Properties of the Essential Oil of Elecampane (Inula helenium L.) Journal of Essential Oil Research. ,vol. 5, pp. 411- 417 ,(1993) , 10.1080/10412905.1993.9698251
Francisco Tomás-Barberán, Esther Iniesta-Sanmartín, Francisco Tomás-Lorente, Angel Rumbero, Antimicrobial phenolic compounds from three Spanish Helichrysum species Phytochemistry. ,vol. 29, pp. 1093- 1095 ,(1990) , 10.1016/0031-9422(90)85410-H