An endogenous artificial microRNA system for unraveling the function of root endosymbioses related genes in Medicago truncatula.
作者： Emanuel A Devers , Julia Teply , Armin Reinert , Nicole Gaude , Franziska Krajinski
关键词: Transformation (genetics) 、 Cell biology 、 Botany 、 Medicago truncatula 、 Gene 、 Heterologous expression 、 Biology 、 Function (biology) 、 Gene silencing 、 microRNA 、 Transcription factor
摘要: Legumes have the unique capacity to undergo two important root endosymbioses: nodule symbiosis and arbuscular mycorrhizal symbiosis. Medicago truncatula is widely used unravel functions of genes during these symbioses. Here we describe development an artificial microRNA (amiR)-mediated gene silencing system for M. roots. The endogenous (miR) mtr-miR159b was selected as a backbone molecule driving amiR expression. Heterologous expression mtr-miR159b-amiR constructs in tobacco showed that functional mediates efficient silencing. amiR-mediated visible marker also effective after transformation constitutively expressing marker. Most importantly, applied novel shed light on function putative transcription factor, MtErf1, which strongly induced arbuscule-containing cells MtPt4 promoter driven amiR-silencing led decreased transcript levels deformed, non-fully truncated arbuscules indicating MtErf1 required arbuscule development. demonstrated here presents highly tool endosymbioses.
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