Scutellarin Alleviates Behavioral Deficits in a Mouse Model of Multiple Sclerosis, Possibly Through Protecting Neural Stem Cells.

摘要: Scutellarin, a flavonoid extracted from an herbal medication (Erigeron breviscapus Hand-Mazz), has been shown to protect neurons against damage and promote neurogenesis, thus therapeutic potential in the treatment of variety neurodegenerative diseases. Since neural stem cells (NSCs) could differentiate into myelin-producing oligodendrocytes, we speculate that scutellarin also be used treat multiple sclerosis (MS). In current study, examined effects using mouse model MS. Briefly, adult C57BL/6 mice exposed cuprizone (8 mg/day through diet, for 6 consecutive weeks) randomly received (50 mg/kg/day) or vehicle 10 days. scutellarin-treated group, rotarod testing at end showed significant improvement motor function (increased time fall); myelin basic protein (MBP) staining corpus callosum revealed decreased demyelination; TUNEL followed by Nestin Sox2 increased number NSCs rate NSC apoptosis subventricular zone (SVZ) lateral ventricles (LV). series experiments cultured subjected injury, confirmed protective scutellarin. At 30 μM, commitment oligodendrocyte neuronal lineages, as evidenced NG2 chondroitin sulfate proteoglycan (NG2) doublecortin (DCX) staining. Differentiation astrocytes (as glial fibrillary acidic (GFAP) staining) was decreased. Maturation committed lineage, marker O4 antibody (O4) MBP staining, promoted Further analysis might suppress phosphorylation p38 cuprizone-induced NSCs. summary, alleviate deficits MS, possibly inhibiting promoting differentiation oligodendrocytes.