Controlling lipase stereoselectivity via the surface pressure.
作者： E. Rogalska , S. Ransac , R. Verger
DOI: 10.1016/S0021-9258(18)54003-5
关键词: Enantiomeric excess 、 Chemistry 、 Gastric lipase 、 Stereochemistry 、 Rhizomucor miehei 、 Triacylglycerol lipase 、 Lipoprotein lipase 、 Candida antarctica 、 Lipase 、 Stereoselectivity
摘要: In the present study, stereoselectivity of Rhizomucor miehei lipase, lipoprotein Candida antarctica B and human gastric lipase towards racemic dicaprin spread as a monolayer at air-water interface was investigated. For this purpose we have developed method with which enantiomeric excess residual substrate can be measured in monomolecular films. The stereoselectivity, is one main aspects enzymic catalysis, found to depend on surface pressure substrate. With all four lipases tested, low pressures enhanced while decreasing enzymes' catalytic activity.
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