Profiling the Sulfation Specificities of Glycosaminoglycan Interactions with Growth Factors and Chemotactic Proteins Using Microarrays

摘要: We report a carbohydrate microarray-based approach for the rapid, facile analysis of glycosaminoglycan-protein interactions. The key structural determinants responsible protein binding, such as sulfate groups that participate in interactions, were elucidated. Specificities also readily compared across families or functional classes, and comparisons among glycosaminoglycan subclasses provided more comprehensive understanding specificity. To validate approach, we showed fibroblast growth factor family members have distinct sulfation preferences. demonstrated heparan chondroitin interact sulfation-dependent manner with various axon guidance proteins, including slit2, netrin1, ephrinA1, ephrinA5, semaphorin5B. anticipate these microarrays will accelerate discovery glycosaminoglycan-binding proteins provide deeper their roles regulating diverse biological processes.