Detection and identification of Mycobacterium tuberculosis by DNA amplification.

摘要: The polymerase chain reaction (PCR) was used to identify mycobacterial DNA sequences in uncultured clinical specimens. Two oligonucleotide primers derived from the sequence of a gene that codes for 65-kilodalton antigen Mycobacterium tuberculosis amplified all 11 species mycobacteria tested. Amplified DNAs nontuberculosis were found be approximately 20 40 bases shorter than those M. and bovis BCG. equivalent present as few cells either alone or presence 10(6) human could detected. Results analysis cultured bacteria specimens showed PCR sensitive specific both detecting differentiating BCG other mycobacteria. method with reported here may become useful tool early rapid detection infections