Degradación in vitro de Bouteloua repens por cultivos de R. flavefaciens y F. succinogenes aislados de ganado alimentado con pastos tropicales en Colombia

摘要: The capacity to degrade the cellular wall of Bouteloua repens of 12 isolates of Ruminococcus flavefaciens and 17 of Fibrobacter succinogenes was determined. A procedure was developed standardize both, inoculum and substrate concentration for in vitro incubations of pure culture isolates.The ruminal bacteria were isolated from either Bos indicus cattle Alto Magdalena region Colombia, grazing Bouteloua repens, Sanmartinero cows (a Colombian criollo breed)  eastern lowlands (Llanos Orientales) grazing Brachiara spp. The identity confirmed using molecular methods. All of F. succinogenes showed a high cell preparations than those of R. flavefaciens (P<0.0001).The effect individual on digestibility also highly significant (P<0.0001). Native surpassed considerably reference strains flavefaciens ATCC 19208 (12.83% vs. 6.83%) and F. succinogenes ATCC 19169 (13.77% 7.94%) their digest Bouteloua repens cellular wall.This difference (p<0.05) for all isolates. Findings suggest that in tropical conditions native has lignocellulose. Further research about potential usefulness specific inocula or enzyme extracts such improving degradability low quality forages by cattle what industrial applications can be improved use these products.