Significant anti-tumour activity of adoptively transferred T cells elicited by intratumoral dendritic cell vaccine injection through enhancing the ratio of CD8+ T cell/regulatory T cells in tumour

摘要: We have shown that immunization with dendritic cells (DCs) pulsed hepatitis B virus core antigen virus-like particles (HBc-VLP) packaging cytosine-guanine dinucleotide (CpG) (HBc-VLP/CpG) alone were able to delay melanoma growth but not eradicate the established tumour in mice. tested whether, by modulating vaccination approaches and injection times, anti-tumour activity could be enhanced. used a B16-HBc murine model only compare efficacy of DC vaccine immunized via footpads, intravenously or intratumoral injections treating priming tumour-specific immune responses, also observe how improve adoptively transferred T induce an enhanced response. Our results indicate that, although all protect mice from developing melanoma, three DCs significant Furthermore, combination adoptive cell transfer led more robust response than use each treatment individually increasing CD8(+) ratio cell/regulatory site. Moreover, induced responses regression protected surviving rechallenge, which is attributed increase CD127-expressing interferon-γ-producing cells. Taken together, these repeated induces expansion antigen-specific against tumour-associated antigens sites, leads elimination pre-established tumours, supporting this combined approach as potent strategy for DC-based cancer immunotherapy.