Differential Staining of Bacteria: Flagella Stain

摘要: Bacterial flagella are appendages used for motility. Their presence is a useful tool identification and differentiation of prokaryotes. Since too thin to be seen by compound light microscopy, staining methods employ the use mordant (often tannic acid) make them thick enough see using an oil immersion objective. Two protocols described. Basic Protocol 1 modified Leifson method one that many microbiologists have adapted. 2 wet-mount stain Ryu included because stable at room temperature. Both these fairly time-consuming, taking from 15 as long 60 min perform.