Chemically characterized concentrated corodies11Corody, pl. Corodies is defined as “an allowance as of food, provision, aliment, etc.”, here it is used in place of “nutrient media”. for continuous cell culture (the 7C's culture media)

摘要: A series of concentrated chemically characterized media has been developed for continuous culture mammalian cells and designated as 7C's media. They contain amino acids at levels from 27 to 162 mM/l comparable the acid content 5 30 per cent serum or 0.3 1.5 lactalbumine hydrolysate, but about times concentrations contained in most defined currently use. The fat-soluble vitamins, polyunsaturated fatty acids, trace elements known be nutritional essentials organism. The proportionality pattern these are, general, similar those found animal tissues food proteins high biological values and, therefore, avoids gross imbalance. The vitamins were based on their potency determined whole organisms taking into consideration nonstorage human tissues. are proportion existing plasma body fluids. The buffering capacity improved compared other CDM due two factors: (1) substitution bicarbonate buffer with β-glycerophosphoric disodium corrects tendency pH rise during incubation because loss CO2 since calcium salt glycerophosphate is soluble water it does not form precipitates like inorganic phosphate buffers; (2) increase zwitterionic character substances [19]. Continuous studies WRC-256 MBIII lymphoblast demonstrate that AN-54 AN-81 able support satisfactory growth cell lines without supplements any tissue extracts. These have maintained serum-free five years, respectively.