Isolation and properties of the DNA of African swine fever (ASF) virus.
作者： L. Enjuanes , A. L. Carrascosa , E. Vinuela
DOI: 10.1099/0022-1317-32-3-479
关键词: Sedimentation coefficient 、 Virus 、 Cell culture 、 Molecular mass 、 Biology 、 Molecular biology 、 Centrifugation 、 DNA 、 Vero cell 、 Haplorhini 、 Virology
摘要: African swine fever (ASF) virus was grown either in macrophages or VERO cells and purified free of cell DNA. Virus DNA isolated from virions as a molecule with sedimentation coefficient 60S contour 58 +/- 3 mum. .these two values give mol. wt. 102 5 X 10(6) 107 10(6), respectively, for the genome ASF virus. Denatured fragments reassociate C0t1/2 value 0-60 0-05 MS, which compared corresponding T4 gives molecular mass 8 daltons. Only is synthesized virus-infected macrophages.
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