Slowing down DNA translocation through a nanopore in lithium chloride.
作者： Stefan W. Kowalczyk , David B. Wells , Aleksei Aksimentiev , Cees Dekker
DOI: 10.1021/NL204273H
关键词: DNA 、 Biophysics 、 Gel electrophoresis 、 Lithium chloride 、 Molecular dynamics 、 Crystallography 、 Molecule 、 Counterion 、 Chemistry 、 A-DNA 、 Nanopore
摘要: The charge of a DNA molecule is crucial parameter in many detection and manipulation schemes such as gel electrophoresis lab-on-a-chip applications. Here, we study the partial reduction due to counterion binding by means nanopore translocation experiments all-atom molecular dynamics (MD) simulations. Surprisingly, find that time through solid-state strongly increases counterions decrease size from K+ Na+ Li+, both for double-stranded (dsDNA) single-stranded (ssDNA). MD simulations elucidate microscopic origin this effect: Li+ bind stronger than K+. These fundamental insights into also provide practical method achieving at least 10-fold enhanced resolution
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